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KMID : 0363420150290040097
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Journal of Korean Oriental Pediatrics
2015 Volume.29 No. 4 p.97 ~ p.107
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The Effects of Hataedock on 2,4-dinitrofluorobenzene Induced Atopic Dermatitis Like Skin Lesion in NC/Nga Mice
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Cha Ho-Yeol
Ahn Sang-Hyun
Jeong A-Ram
Cheon Jin-Hong
Park Sun-Young
Kim Ki-Bong
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Abstract
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Objectives: Hataedock is the treatment that dispels toxic heat and meconium gathered at the fetus for the new born baby by orally administered herbal extracts. The purpose of this study was to evaluate whether Hataedock alleviate inflammatory skin damages in AD-induced NC/Nga mice through regulating of skin barrier maintain and Th2 differentiation.
Methods: We established an AD model in the 3-week-old NC/Nga mice through the repeated application of DNFB (dinitrochlorobenzene) on days 28, 35, 42 after Hataedock treatment which was orally administered. We identified the changes of skin barrier and Th2 differentiation through the histological and immunohistochemical changes of protein kinase C (PKC), interleukin (IL)-4, degranulated mast cell, Substance P and MMP-9.
Results: Our results suggested that Hataedock treatment significantly down-regulated levels of PKC by 82% (p < 0.001), as well as IL-4 by 56% (p < 0.001). Hataedock also suppressed mast cell infiltration, ear edema formation. and Substance P in the tissue of NC/Nga mice were decreased by 57% (p < 0.001), and MMP-9 by 55% (p < 0.001).
Conclusions: These results suggest that Hataedock alleviates AD through the down-regulation of PKC and Th2 cytokines, which are involved in the initial steps of AD development. Hataedock have potential application for the treatment of AD.
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KEYWORD
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Hataedock, Atopic dermatitis, PKC, IL-4, NC/Nga mouse, DNFB
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